RNA isolation / purification

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - HUVEC

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U266

Get tips on using Naive Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U-87 MG

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T