Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse Human

Get tips on using CD4 Monoclonal Antibody (OKT4 (OKT-4)), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD4

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using CD61-FITC, SZ21, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using CD22-PE, SJ10.1H11, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD22

Get tips on using CD30-PE, HRS4, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD30

Get tips on using CD41-PE, P2, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD41