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Get tips on using Mammalian Cell Lysis kit to perform Protein isolation Mammalian cells - STTG1

Products Sigma-Aldrich Mammalian Cell Lysis kit

Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - STTG1

Products Merck Millipore RIPA Lysis Buffer, 10X

Get tips on using TRI Reagent™ Solution to perform Protein isolation Bacteria - Vibrio cholerae

Products Thermo Fisher Scientific TRI Reagent™ Solution

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells polymer / lipid

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Products Qiagen AllPrep DNA/RNA Micro Kit

Proteins Protein expression and purification Mammalian cells HEK 293 HER2 leader peptide

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2

Products Roche Lifesciences VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody

Get tips on using Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO) to perform Immunohistochemistry Human - MLH1

Products BD Biosciences Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO)

Get tips on using Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441 to perform Immunohistochemistry Human - AR

Products Agilent Technologies Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8 to perform Immunohistochemistry Human - CK20

Products Agilent Technologies Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8

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