siRNA / miRNA gene silencing Human OV2008 Yap Gene

Get tips on using MACSprep™ HLA T Cell Isolation Kit, human to perform Cell Isolation HLA T Cell

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation CD20

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation ERBB2

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion FUT8

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion TRIM25

Get tips on using GeneJET RNA Purification Kit to perform RNA isolation / purification Cells - primary human renal artery smooth muscle cells

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Insertion IMR-32 SULT4A

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Point mutation U2OS PCNA

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Point mutation LNCaP RasGRP2