siRNA / RNAi /miRNA transfection Rat

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized T98G

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PNT2

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized DU145

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U-251