siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

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The kit works good in human tissue biopsy samples even with minimum amount of tissue.

Discussions is it suitable for human adipose tissue biopsy samples.

Get tips on using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 to perform Immunohistochemistry Human - E-Cadherin

Products Agilent Technologies Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

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Get tips on using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion to perform Cell Isolation Monocyte

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Get tips on using Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD23

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Get tips on using EasySep™ Direct Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Products STEMCELL technologies EasySep™ Direct Human Naïve B Cell Isolation Kit

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

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Get tips on using CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Products Miltenyibiotec CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using MACSxpress Whole Blood Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell

Products Miltenyibiotec MACSxpress Whole Blood Pan T Cell Isolation Kit, human

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