ChIP H3K27me3 Rat Human

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using CD38 CRISPR Activation Plasmid (r) to perform CRISPR Rat - Activation CD38

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Immunohistochemistry Rat - Sox9

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.