ChIP acH3 Human Rat

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Rat - Endothelin 1

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using CD38 CRISPR Activation Plasmid (r) to perform CRISPR Rat - Activation CD38

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Immunohistochemistry Rat - Sox9

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

Get tips on using 17 beta Estradiol ELISA Kit (ab108667) to perform ELISA Rat - Estradiol

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.