DNA isolation / purification Cells primary cells

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media NCH421K cells primary glioma

Get tips on using mirVana® miRNA mimic to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells hsa-miR-542-3p

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Rat Pancreas

Get tips on using MicroRNA isolation kit to perform RNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using Zymoclean™ Gel DNA Recovery Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Clostridium sporogenes