siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human primary breast ephitelial cells-organoids

Get tips on using Target sequence1: Seq1-5'-GACTTCACGGGTGGTGTTTCT-3' to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Torin 1 to perform Autophagy assay cell type - Mel cells

Get tips on using Dynabeads™ Untouched™ Human B Cells Kit to perform Cell Isolation B cell

Get tips on using Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Rat Kidney injury molecule 1,Kim-1 ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Rat - KIM-1