Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells
Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary canine aortic endothelial cells
Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary lamb pulmonary artery endothelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental venous endothelial cells
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