How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Get tips on using RNeasy 96 Universal Tissue Kit (4) to perform RNA isolation / purification Tissue - Mouse Kidney
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle
Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse lung tissue
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse brain tissue
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - mouse adipose tissue
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse liver tissue
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