siRNA / RNAi /miRNA transfection Human Cells OV-2008

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary mesenchymal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary epithelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human skeletal muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental epithelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human marrow stromal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.