protein-isolation-bacteria-salmonella-typhi

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus helveticus

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus plantarum

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using BBL™ Brain Heart Infusion Broth, BD to perform Bacterial cell culture media Clostridum botulinum

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Clostridium difficile

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Bacillus cereus

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Bacillus anthracis

Get tips on using Luria Bertani Broth, Miller (Miller Luria Bertani Broth) to perform Bacterial cell culture media Escherichia coli

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.