Site Directed Mutagenesis (SDM) Monkey Point mutation Cos-7

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using HiPerFect Transfection Reagent (100 ml) to perform siRNA / RNAi /miRNA transfection Bovine - monocyte-derived macrophages

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using In situ apoptosis detection to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using CardioTACS™ In Situ Apoptosis Detection Kit to perform Apoptosis assay cell type - Cardiomyocytes

Get tips on using In Situ Cell Proliferation Kit, FLUOS to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC