CRISPR Rat Deletion INS-1 832/13

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Ramos

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained

Get tips on using Blue Prestained Protein Standard, Broad Range (11-250 kDa) to perform Protein Ladder Prestained

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - MCF7

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - A172

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - A375

Get tips on using Cellular ROS/Superoxide Detection Assay Kit to perform ROS assay cell type - Raw 264.7

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - RAW 264.7

Get tips on using OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY to perform Immunohistochemistry Mouse - Spp1/OPN