Protein expression and purification Insect cells

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary bovine aortic endothelial cells

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human primary mammary adipose derived stem cells

Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Brain tissue from rhesus monkey Biotin

Get tips on using D-MEM (High Glucose) with L-Glutamine and Phenol Red to perform Stem cell culture media hTrophoblasts

Get tips on using D-MEM (High Glucose) with L-Glutamine and Phenol Red to perform Mammalian cell culture media HLF

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human aortic endothelial cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.