RNA isolation / purification Cells immortalized

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to be considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using EasySep™ Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human Pan Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using Slan (M-DC8)+ Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using EasySep™ Direct Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using Naive B Cell Isolation Kit II, human to perform Cell Isolation Naive B cell

Get tips on using Naive Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell