dna-methylation-profiling-gene-specific-profiling-hypothalamus-mouse-tissue-mecp2

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Get tips on using E.Z.N.A.® BAC/PAC DNA-kits to perform Plasmid Isolation Acinetobacter towneri

Get tips on using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - U87 and U251 glioblastoma cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

Get tips on using single stranded-DNA Apoptosis ELISA kit to perform Apoptosis assay cell type - A2780

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using MasterPure™ Complete DNA and RNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - HT-29