shRNA gene silencing Mouse RGC-5

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - NIH3T3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using TransMessenger Transfection Reagent (0.5 ml) to perform siRNA / RNAi /miRNA transfection Mouse - Primary cortical and hippocampal cell

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Get tips on using Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix to perform 3D Cell Culture Media Mouse fallopian organoids

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse tracheal epithelial cells

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse cardiac fibroblasts

Get tips on using ATG5 Antibody Novus to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid