ChIP acH4 Chicken Canine

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Mre11 Antibody #4895 to perform ChIP Anti-bodies MRE11

Get tips on using Anti-CTCF Antibody to perform ChIP Anti-bodies CTCF

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Mouse - Osteoblasts

Get tips on using Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) to perform ChIP Anti-bodies CtIP/BCL11A

Get tips on using Histone H3K9ac antibody to perform ChIP H3K9Ac - Sheep Rat -NA-

Get tips on using Histone H2A.X antibody (pAb) to perform ChIP Anti-bodies γH2AX

Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1

Get tips on using Histone H3K36me3 antibody (mAb) to perform ChIP Anti-bodies H3K36me3