Get tips on using QIAzol Lysis Reagent to perform RNA isolation / purification Tissue - rat lung tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat lung tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - human lung tissue
Get tips on using RIPA Lysis Buffer (ProteinSimple) to perform Protein isolation Tissue - Mouse cardiac tissue
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Liver
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Kidney
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