ROS assay cell type

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - H9C2

Get tips on using X-Gal Staining Kit to perform Reporter gene assay β-galactosidase substrates - C2C12

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - Huh7

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U-87 MG

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Escherichia coli

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Corynebacterium glutamicum

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Bacillus subtilis

Get tips on using LIVE/DEAD™ Yeast Viability Kit to perform Live / Dead assay yeast - Urediniospore

Get tips on using Zombie Aqua™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - human peripheral blood mononuclear cells

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.