Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus
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Get tips on using QIAamp DSP DNA Blood Mini Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using EZ1 DSP DNA Blood Kit (48) to perform DNA isolation / purification Tissue - blood / plasma
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A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
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