rna-isolation-purification-tissue-mouse-spine

Get tips on using APC anti-mouse CD206 (MMR) Antibody to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using Mouse Integrin alpha E/CD103 Antibody to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using PE/Cyanine7 anti-mouse CD14 Antibody to perform Flow cytometry Anti-bodies Mouse - CD14

Get tips on using Mouse CD200 R3 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - CD200

Get tips on using Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) to perform ELISA Mouse - Serpin E1/PAI-1

Get tips on using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit to perform ELISA Mouse - RANK L

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - Neuro 2a

Get tips on using FoxP3 Antibody, anti-mouse, PE, REAfinity™ to perform Flow cytometry Anti-bodies Mouse - FOXP3