Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

Get tips on using Anti-CRISP3 antibody (ab105951) to perform Immunohistochemistry Human - CRISP3

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - AGS

Get tips on using Re-ChIP-IT® to perform ChIP Human - LCL

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using Silencer select_ObR siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 ObR(leptin receptor/LEPR)

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.