dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using QIAamp DSP Virus Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using Pan T Cell Isolation Kit, human to perform Cell Isolation Human T cells

Get tips on using QIAGEN Genomic-tip 20/G to perform DNA isolation / purification Plants - Leaves

Get tips on using EZ1 Virus Mini Kit v2.0 (48) to perform DNA isolation / purification Viral

Get tips on using QIAamp MinElute Virus Spin Kit (50) to perform DNA isolation / purification Viral

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - Rat fibroblast-like synoviocytes

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media NCH421K cells primary glioma

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine