RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using Purified Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135
Get tips on using PE Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135
Get tips on using PE Mouse Anti-Human CD122 to perform Flow cytometry Anti-bodies Human - CD122
Get tips on using PE Mouse Anti-Human CD116 to perform Flow cytometry Anti-bodies Human - CD116
Get tips on using PE Mouse Anti-Human CD114 to perform Flow cytometry Anti-bodies Human - CD114
Get tips on using PE Mouse anti-Human CD96 to perform Flow cytometry Anti-bodies Human - CD96
Get tips on using Purified Mouse Anti-Human CD61 to perform Flow cytometry Anti-bodies Human - CD61
Get tips on using CD52 Monoclonal Antibody (CF1D12), PE to perform Flow cytometry Anti-bodies Human - CD52
Get tips on using PE Mouse anti-Human CD52 to perform Flow cytometry Anti-bodies Human - CD52
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment