Get tips on using X-Gal Staining Kit to perform Reporter gene assay β-galactosidase substrates - C2C12
Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - Huh7
Get tips on using MCM4 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - SiHa MCM4
Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1
Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Rab Coupling Protein (RCP)
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells
Get tips on using Arp2 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D Arp-2
Get tips on using siRNA TICAM-1 to perform siRNA / miRNA gene silencing Human - HT-29 TICAM-1
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - MRC-5 BARX1
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