Protein expression and purification Insect cells S2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A431

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A253

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.