rna-isolation-purification-cells-primary-porcine-primary-airway-epithelial-cell

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse muscle tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human breast tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human placental tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human aorta tissue

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Rat Kidney

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Thyroid gland

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Spinal cord