ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Liver
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Veins
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Tonsil
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Prostate
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Muscles
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Liver
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