siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using pGEX-4T-1 to perform Protein Expression Prokaryotic cells - E. coli LsrK

Get tips on using Torin 1 to perform Autophagy assay cell type - Mel cells

Get tips on using Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Rat Kidney injury molecule 1,Kim-1 ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

Get tips on using Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

Get tips on using pPG-1-FlaA to perform Protein Expression Prokaryotic cells - E. coli surface flagellin A

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.