CRISPR Hamster Deletion CHO-K1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using pLV-EF1a-IRES-Puro to perform CRISPR Mouse - Deletion L929 Ppm1b

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Get tips on using pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W to perform CRISPR Human - Deletion KAT2A

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion PKR

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion AURKB

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion GATA1

Get tips on using CMV-CAS9-2A-GFP Plasmid to perform CRISPR Human - Deletion TRIB1

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Deletion NOX4