Site Directed Mutagenesis (SDM) Mouse L929

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - L929

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - L929

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - L929

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - L929

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - L929

Get tips on using FITC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using APC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1