rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Intestine

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Brain

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Rat Subcutaneous

Get tips on using Dual-Luciferase® Reporter Assay System to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Get tips on using QIAzol Lysis Reagent to perform RNA isolation / purification Tissue - rat lung tissue