rna-isolation-purification-tissue-rat-sublingual-glands

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA, rhesus monkey brain tissue Biotin

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - rat mammary tissue

Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - rat renal cortex tissue

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - rat kidney and pancreas tissue

Get tips on using MagNA Pure Compact Nucleic Acid Isolation Kit I to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae