Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - Human gingival epithelial cells
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - THP-1
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - KC02-44D
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