Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse brain tissue Biotin
Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin
Get tips on using NucleoSpin® Gel and PCR Clean-up to perform DNA gel extraction / PCR product purification Product size > 15Kb
Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells
Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells
There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
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