Western blot LC3B Rabbit Human

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - OVCAR-5

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - HL-60

Get tips on using FITC BrdU Flow Kit to perform Cell cycle assay human - THP-1

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Phalloidin–Tetramethylrhodamine B isothiocyanate to perform FACS Phalloidin - Amanita phalloides Human TRITC

Get tips on using PerCP-Cy5-labeled CD66b to perform FACS CD66b - Mouse Human PerCP-Cy5

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Anti-Glucocorticoid Receptor antibody (ab3578) to perform Immunohistochemistry Human - GR/glucocorticoid receptor