siRNA / miRNA gene silencing Human HNSCC cell line

Get tips on using Astrocyte Medium to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin

Get tips on using Human BDNF ELISA Kit (ab212166) to perform ELISA Mouse - BDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Mouse - BDNF

Get tips on using Human SCF ELISA Kit (ab100636) to perform ELISA Rat - SC

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Human VWF / von Willebrand Factor ELISA Kit to perform ELISA Human - VWF-A2

Get tips on using Human TNF Alpha PicoKine™ ELISA Kit to perform ELISA Human - TNF-alpha