Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta
Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Rat_Liver
Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Huh7
Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7
Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - HUVEC
Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin
Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines COS7
Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa
Get tips on using Fluorescent 1 kb DNA Ladder to perform DNA Ladder 1 kb
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