siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using Human VE-Cadherin Quantikine ELISA Kit to perform ELISA Human - VE Cadherin

Get tips on using Human TNF alpha ELISA Kit (ab181421) to perform ELISA Human - TNF-alpha

Get tips on using Human TNF-alpha Quantikine ELISA Kit to perform ELISA Human - TNF-alpha

Get tips on using Human PDGF BB ELISA Kit (ab100624) to perform ELISA Human - PDGF-BB

Get tips on using Human Cytochrome c Quantikine ELISA Kit to perform ELISA Human - Cytochrome C

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using PE anti-human CD96 (TACTILE) Antibody to perform Flow cytometry Anti-bodies Human - CD96

Get tips on using FITC Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51