rna-isolation-purification-cells-immortalized-mda-mb-231

Get tips on using QIAamp DSP DNA Blood Mini Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using EZ1 DSP DNA Blood Kit (48) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Blood Mini QIAcube Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using RPM Yeast Plasmid Isolation Kit, 100 preps to perform Plasmid Isolation S. cerevisiae

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U266

Get tips on using EasySep™ Direct Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human B Cell (CD43-) Isolation Kit to perform Cell Isolation B cell

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using MojoSort™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell