Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD4+ T cells
Get tips on using QuikChange Multi Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation BxPC-3 uPAR
Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation U-87MG Rictor
Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation SH-SY5Y Rab1
Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62
Get tips on using Human MMP-1 Antibody to perform Western blotting MMP1
Get tips on using ON-TARGETplus Human PPRC1 siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PRC (PGC-1α–related coactivator)/PPRC1
Get tips on using Aurum™ Total RNA Fatty and Fibrous Tissue Kit to perform RNA isolation / purification Tissue - Human Uterus
Get tips on using Aurum™ Total RNA Fatty and Fibrous Tissue Kit to perform RNA isolation / purification Tissue - Human Adipose
Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.
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