RNA isolation / purification Tissue Human

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Dynabeads™ CD34 Positive Isolation Kit to perform Cell Isolation CD34+ cells

Get tips on using MicroRNA isolation kit to perform Test Lionel Test Lionel - Test Lionel Test Lionel Test Lionel Test Lionel Test Lionel

Get tips on using NucleoSpin® Soil to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using Genomic DNA MiniSpin: Bacteria to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using Oligotex mRNA Midi Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using Oligotex mRNA Mini Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA