CRISPR Mouse Deletion

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I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression Mstn

Products Addgene lentiCRISPR v2

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression CCR-5

Products Addgene lentiCRISPR v2

Get tips on using pLKO5.sgRNA.EFS.GFP to perform CRISPR Mouse - Activation Neuro-2a Smn1

Products Addgene pLKO5.sgRNA.EFS.GFP

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion C2C12 DMD

Products Thermo Fisher Scientific Phusion Site-Directed Mutagenesis Kit

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Products Addgene pLKO.1 puro

Get tips on using QuikChange Lightning Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion L929 ICP6

Products Agilent Technologies QuikChange Lightning Site-Directed Mutagenesis Kit

Get tips on using pcDNA-mC/EBPb to perform CRISPR Mouse - Activation 3T3-L1 C/EBPβ

Products Addgene pcDNA-mC/EBPb

Get tips on using lenti sgRNA(MS2)_zeo backbone to perform CRISPR Mouse - Activation C2C12 FST

Products Addgene lenti sgRNA(MS2)_zeo backbone

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Activation C2C12 Lama1

Products Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9

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