Cell cycle assay human

Get tips on using ATG5 Antibody Novus to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-SQSTM1 / p62 antibody to perform Autophagy assay cell type - MDA-MB-231

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using LAMP-1 Antibody (H4A3) to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - NRK-52E

Get tips on using Anti-Beclin 1 antibody (ab62557) to perform Autophagy assay cell type - NRK-52E