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Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HL-1

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - J774A.1

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - CAKI-1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - THP 1

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - THP-1

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - PANC-1

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Hepa-1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using DNeasy PowerBiofilm Kit (50) to perform DNA isolation / purification Biofilm samples