Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human epidermal keratinocytes
Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis
Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells
Get tips on using SENSE mRNA-Seq Library Prep Kit V2 to perform RNA sequencing Human - Glioblastoma stem-like cells (GSCs)
Get tips on using EZ-96 DNA Methylation-Gold™ Kit to perform DNA methylation profiling Whole genome profiling - human placenta
Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human epithelial cells
Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human prostate organoid
Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line
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